Original Research

Published on 20 Jan 2022

Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)

in Genome Editing in Plants

Frida Meijer Carlsen
Ida Elisabeth Johansen
Zhang Yang
Ying Liu
Ida Nøhr Westberg
Nam Phuong Kieu
Bodil Jørgensen
Marit Lenman
Erik Andreasson
Kåre Lehmann Nielsen

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.795644

4,214 views
14 citations

Original Research

Published on 12 Jan 2022

Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

in Genome Editing in Plants

Raviraj Banakar
Mollie Schubert
Gavin Kurgan
Krishan Mohan Rai
Sarah F. Beaudoin
Michael A. Collingwood
Christopher A. Vakulskas
Kan Wang
Feng Zhang

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.760820

8,945 views
20 citations

Original Research

Published on 22 Dec 2021

A Versatile and Efficient Plant Protoplast Platform for Genome Editing by Cas9 RNPs

in Genome Editing in Plants

Wenzhi Jiang
Jenifer Bush
Jen Sheen

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.719190

7,794 views
13 citations

Mini Review

Published on 17 Dec 2021

Targeted Gene Knockouts by Protoplast Transformation in the Moss Physcomitrella patens

in Genome Editing in Plants

Lei Zhu

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.719087

6,125 views
3 citations

Mini Review

Published on 15 Dec 2021

Non-GM Genome Editing Approaches in Crops

in Genome Editing in Plants

Zheng Gong
Ming Cheng
Jose R. Botella

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.817279

12,339 views
28 citations

Mini Review

Published on 02 Dec 2021

Transgene-free Genome Editing in Plants

in Genome Editing in Plants

Xiaoyong Gu
Lijing Liu
Huawei Zhang

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.805317

25,414 views
44 citations

Original Research

Published on 29 Nov 2021

Targeted Knockout of Eukaryotic Translation Initiation Factor 4E Confers Bymovirus Resistance in Winter Barley

in Genome Editing in Plants

Robert Eric Hoffie
Ingrid Otto
Dragan Perovic
Nagaveni Budhagatapalli
Antje Habekuß
Frank Ordon
Jochen Kumlehn

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.784233

3,721 views
24 citations

Original Research

Published on 16 Nov 2021

Establishment of an Efficient Protoplast Regeneration and Transfection Protocol for Field Cress (Lepidium campestre)

in Genome Editing in Plants

Sjur Sandgrind
Xueyuan Li
Emelie Ivarson
Annelie Ahlman
Li-Hua Zhu

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.757540

4,500 views
5 citations

Editorial

Published on 08 Oct 2021

Editorial: New Genome Editing Tools and Resources: Enabling Gene Discovery and Functional Genomics

in Genome Editing in Plants

Wendy Harwood
Qi-Jun Chen
Feng Zhang
Bing Yang

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.771622

2,208 views
1 citation

Original Research

Published on 15 Sep 2021

Exploring C-To-G Base Editing in Rice, Tomato, and Poplar

in Genome Editing in Plants

Simon Sretenovic
Shishi Liu
Gen Li
Yanhao Cheng
Tingting Fan
Yang Xu
Jianping Zhou
Xuelian Zheng
Gary Coleman
Yong Zhang

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.756766

6,502 views
41 citations

Editorial

Published on 09 Sep 2021

Editorial: Targeted Genome Editing in Crops

in Genome Editing in Plants

Huanbin Zhou
Yong Zhang
Lanqin Xia
Seiichi Toki

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.757916

1,406 views

Review

Published on 03 Sep 2021

Protoplast Regeneration and Its Use in New Plant Breeding Technologies

in Genome Editing in Plants

Kelsey M. Reed
Bastiaan O. R. Bargmann

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.734951

41,285 views
86 citations

Mini Review

Published on 11 Aug 2021

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

in Genome Editing in Plants

Jin-Jun Yue
Jin-Ling Yuan
Fu-Hui Wu
Yu-Hsuan Yuan
Qiao-Wei Cheng
Chen-Tran Hsu
Choun-Sea Lin

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.717017

16,958 views
53 citations

Original Research

Published on 09 Aug 2021

Loss of MAR1 Function is a Marker for Co-Selection of CRISPR-Induced Mutations in Plants

in Genome Editing in Plants

Jannis Rinne
Claus-Peter Witte
Marco Herde

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.723384

3,801 views
11 citations

Original Research

Published on 08 Jul 2021

CRISPR/Cas9-Mediated Multi-Allelic Gene Targeting in Sugarcane Confers Herbicide Tolerance

in Genome Editing in Plants

Mehmet Tufan Oz
Angelika Altpeter
Ratna Karan
Aldo Merotto
Fredy Altpeter

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.673566

16,882 views
72 citations

Original Research

Published on 15 Jun 2021

In-planta Gene Targeting in Barley Using Cas9 With and Without Geminiviral Replicons

in Genome Editing in Plants

Tom Lawrenson
Alison Hinchliffe
Martha Clarke
Yvie Morgan
Wendy Harwood

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.663380

6,067 views
11 citations

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Articles

Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)

Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

A Versatile and Efficient Plant Protoplast Platform for Genome Editing by Cas9 RNPs

Targeted Gene Knockouts by Protoplast Transformation in the Moss Physcomitrella patens

Non-GM Genome Editing Approaches in Crops

Transgene-free Genome Editing in Plants

Targeted Knockout of Eukaryotic Translation Initiation Factor 4E Confers Bymovirus Resistance in Winter Barley

Establishment of an Efficient Protoplast Regeneration and Transfection Protocol for Field Cress (Lepidium campestre)

Editorial: New Genome Editing Tools and Resources: Enabling Gene Discovery and Functional Genomics

Exploring C-To-G Base Editing in Rice, Tomato, and Poplar

Editorial: Targeted Genome Editing in Crops

Protoplast Regeneration and Its Use in New Plant Breeding Technologies

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

Loss of MAR1 Function is a Marker for Co-Selection of CRISPR-Induced Mutations in Plants

CRISPR/Cas9-Mediated Multi-Allelic Gene Targeting in Sugarcane Confers Herbicide Tolerance

In-planta Gene Targeting in Barley Using Cas9 With and Without Geminiviral Replicons